Kinomics accelarating biosensors

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Literature
Kinomics defined beneficial, unique add-ons for affinity-based biosensors.

Kinomics MSK® Test Procedure

Use your existing affinity biosensor and, if possible, a planar sensor chip surface, a high flow rate, a data rate of 1 Hz or higher, a stable capture assay for ligand immobilization, and purified ligand and analyte that should bind to each other in a single-exponential fashion. Prepare up to 10 analyte sample solutions, each doubled in concentration and starting, for example, at 1 nmol/L; the majority of the concentrations should be higher than the expected dissociation equilibrium constant. Immobilize the ligand at a fairly low level on the sensor chip (yielding the first, active surface) and prepare a second, inactive sensor surface for referencing. Run the analyte samples, one after the other and starting with the lowest concentration, over both surfaces - each association for about 2 to 5 min and each binding followed by a 2 min dissociation phase. Reference out drifts and buffer shifts by subtracting the reference channel from the active channel. Save the referenced signal as text file (in tab- or comma-separated format, decimal sign = point) with two columns specifying time and response. Save a second text file with two columns specifying the starting time of each step (unit: s) and the analyte concentration applied in each association and dissociation step (unit: mol/L, i.e., enter 1.00E-09 for 1 nmol/L (1 nM) and just 0 for dissociations).

Published online 09/12/2005

Please, mail both your files to info[a]kinomics.com or contact Kinomics, if indicated, for a more detailed procedure of your specific test.

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Last updated: 01/22/2017
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